KPV / GHK-Cu Blend — wholesale 10-vial kits

Research context

This product combines two tripeptides in one lyophilized vial: KPV, the C-terminal Lys-Pro-Val tripeptide of alpha-melanocyte-stimulating hormone, and GHK-Cu, the copper(II) complex of glycyl-L-histidyl-L-lysine. The supplier listing states the split as 10 mg of KPV and 50 mg of GHK-Cu per vial, 60 mg total, so that composition is stated here; the lot COA confirms each component's identity, purity and content independently of the label.

The two are paired because they sit on different but adjacent arms of tissue-remodeling research. GHK-Cu has the longer record: the glycyl-histidyl-lysine tripeptide was first described in 1973 in Biochemical and Biophysical Research Communications as a factor increasing survival of cultured normal liver cells, and a 2008 review in the Journal of Biomaterials Science collects the matrix-remodeling and connective-tissue literature built on it; its copper-coordination chemistry is central, because the copper(II) adduct, not the metal-free peptide, is the species most of that work describes. KPV is the much smaller signaling tripeptide: structure–activity work on α-MSH separated the hormone's pigmentary pharmacophore from an anti-inflammatory activity that survives in this three-residue C-terminal fragment, which in cell models suppresses NF-κB and MAP-kinase activation and pro-inflammatory cytokine output and enters epithelial cells through the PepT1 transporter. Grouping a matrix-active copper peptide with an anti-inflammatory tripeptide is the kind of comparative or combination design these are normally used for in cell and tissue models.

Pre-blended material has three practical advantages. The ratio is fixed at manufacture, so it is consistent from vial to vial. Each vial needs one reconstitution instead of two. And one lot COA covers both molecules: LC-MS identity is confirmed for each component, and RP-HPLC purity is reported for each component, under the same lot code.

What to check on the certificate, component by component. For GHK-Cu: that identity is reported for the copper(II) complex rather than the free apo-GHK peptide — the copper adduct and the metal-free peptide differ in mass and in UV–visible absorbance, and copper stoichiometry is the usual point of confusion. For KPV: the mass against the Lys-Pro-Val tripeptide; because it is so small, the counter-ion is a large fraction of the weighed mass, so the net peptide content matters when the real ratio in the vial is calculated. For both: individual per-peak RP-HPLC purity rather than a blend total, the counter-ion and net content for each, and the stated amounts — 10 mg KPV and 50 mg GHK-Cu — against the label.

Regulatory position, stated factually: neither component is an FDA-approved drug, and this blend is not a drug product. FDA lists GHK-Cu and KPV among bulk drug substances nominated for compounding, with several nominations withdrawn by the nominators; the HHS interim enforcement-discretion notice published in the Federal Register on October 30, 2026 addresses certain peptide bulk substances in 503A compounding pending rulemaking and is addressed to licensed compounders, not research suppliers, and is not an approval. Component amounts are stated on the lot COA. WholesalePeptide.us supplies this blend as a research reference material for in-vitro, analytical and preclinical laboratory research.

GHK-Cu gives the lyophilized cake and the reconstituted solution a blue tint, which is normal and comes from the copper complex. For a ratio other than the fixed 10 mg / 50 mg split, or for each tripeptide alone as a comparator, order KPV and GHK-Cu separately and combine them in your lab.

Research areas

• skin and connective-tissue remodeling models
• NF-κB and cytokine signaling in epithelial cell models
• copper-peptide coordination chemistry
• collagen and extracellular-matrix synthesis assays
• multi-component LC-MS identity testing
• per-component counter-ion and net-peptide-content checks

Handling and storage

• Store lyophilized vials at −20 °C, sealed, protected from light and moisture.
• Let the vial equilibrate to room temperature before opening to limit condensation.
• Reconstitute in an appropriate sterile buffer or solvent according to your laboratory SOP.
• The blue tint comes from the GHK-Cu copper complex. Reconstitute the whole vial once; both components are then in solution together. Aliquot and avoid repeated freeze–thaw.